Microfluidic cell culture,
evolution and sorting
system
evolution and sorting
system
MMC
Product overviewProduct parameters
Successful cases
Product overview
Microbial Microdroplet Culture system (MMC）is a miniaturized,
automated and intelligent high-throughput microbial culture instrument
developed based on droplet microfluidic technology. A single microfluidic chip
contains about 0~200 droplet culture units, and the volume of each droplet unit
is about 2-3μl. It can carry out 350~800nm full wavelength scanning and
fluorescence excitation detection on the droplets (optional). During the
culture process, the growth of microorganisms (OD detection range up to 15) and
the change of fluorescence intensity (0-60000 counts) can be detected online at
the same time. It can realize automatic subculture, accompanied by multi
gradient addition of chemical factors. Continuous culture time up to 15 days
(or 100 generations). After culture, automatic strain sorting can be carried
out according to the growth status.
Microbial Microdroplet Culture system (MMC）is a miniaturized,
automated and intelligent high-throughput microbial culture instrument
developed based on droplet microfluidic technology. A single microfluidic chip
contains about 0~200 droplet culture units, and the volume of each droplet unit
is about 2-3μl. It can carry out 350~800nm full wavelength scanning and
fluorescence excitation detection on the droplets (optional). During the
culture process, the growth of microorganisms (OD detection range up to 15) and
the change of fluorescence intensity (0-60000 counts) can be detected online at
the same time. It can realize automatic subculture, accompanied by multi
gradient addition of chemical factors. Continuous culture time up to 15 days
(or 100 generations). After culture, automatic strain sorting can be carried
out according to the growth status.
Product parameters
Successful cases
Case 1：Screening of Rhodococcus equine mutants with high CDA production
Through the simultaneous detection of bacterial growth (OD) and enzyme activity (color reaction) at 600nm and 400nm, the sorted mutant strain B4 produced the maximum enzyme of 419.11 U / ml after 36 h fermentation, which was 3.90 times that of the control 107.58 U / ml. (China Brewing, 2020, v.39; No. 342 (08): 174-178.)
Case 2：Screening of
high concentration sorbose resistant Escherichia coli
MMC adaptive evolution culture; A single round of continuous culture for about 120 h and subculture for nearly 20 times; E. coli sorbose tolerance increased by 200%. Improve substrate tolerance and reduce process control cost. (food and fermentation industry, 2020)
Case 3：Adaptive
evolution of methanotrophic bacteria with high tolerance to methanol
The strains with significantly enhanced growth in 5% methanol were screened. （Biotechnology and Bioengineering，2020）
Case 4：adaptive
evolution strategy to enhance the performance of methanol utilizing Escherichia
coli
Through MMC adaptive evolution experiment, a mutant MESV2.2-3 was obtained, which increased the growth rate by 3 times, shortened the lag period by 43%, and reduced the gluconate consumption by 40%.Whole genome sequencing analysis showed that gene gntU,idnT,edd and pckA were mutated, and these genes were closely related to gluconate absorption and cell growth. （Frontiers in Bioengineering and Biotechnology，2020）
Case 1：Screening of Rhodococcus equine mutants with high CDA production
Through the simultaneous detection of bacterial growth (OD) and enzyme activity (color reaction) at 600nm and 400nm, the sorted mutant strain B4 produced the maximum enzyme of 419.11 U / ml after 36 h fermentation, which was 3.90 times that of the control 107.58 U / ml. (China Brewing, 2020, v.39; No. 342 (08): 174-178.)
Case 2：Screening of
high concentration sorbose resistant Escherichia coli
MMC adaptive evolution culture; A single round of continuous culture for about 120 h and subculture for nearly 20 times; E. coli sorbose tolerance increased by 200%. Improve substrate tolerance and reduce process control cost. (food and fermentation industry, 2020)
Case 3：Adaptive
evolution of methanotrophic bacteria with high tolerance to methanol
The strains with significantly enhanced growth in 5% methanol were screened. （Biotechnology and Bioengineering，2020）
Case 4：adaptive
evolution strategy to enhance the performance of methanol utilizing Escherichia
coli
Through MMC adaptive evolution experiment, a mutant MESV2.2-3 was obtained, which increased the growth rate by 3 times, shortened the lag period by 43%, and reduced the gluconate consumption by 40%.Whole genome sequencing analysis showed that gene gntU,idnT,edd and pckA were mutated, and these genes were closely related to gluconate absorption and cell growth. （Frontiers in Bioengineering and Biotechnology，2020）
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