ARTP-IIIS

Product overview

Compared with the traditional low-pressure gas discharge plasma source, atmospheric pressure room temperature plasma (ARTP) has the characteristics of low plasma jet temperature, uniform discharge and high concentration of chemically active particles. Based on ARTP technology, Our company, together with the Department of chemical engineering and the Department of Engineering Physics of Tsinghua University, has jointly developed the first special instrument for mutation and breeding of microorganisms by means of plasma — ARTP Mutagenesis Breeding Machine. The instrument has high mutation rate, compact structure, simple operation, high safety and fast mutation speed. A large mutation library can be obtained by one mutation operation (within a few minutes), which greatly improves the intensity of strain mutation and the capacity of mutation library; ARTP technology combined with high-throughput screening technology can realize rapid and efficient evolutionary breeding of organisms.

Product parameters

Successful cases

Case 1：Screening of High Salt Tolerant Mutant Strains by ARTP

"After plasma treatment, when Enterobacter cloacae was cultured in the medium containing 7.5% NaCl, a salt tolerant mutant appeared, and the degradation percentage of TPH was 2.5 times higher than that of the original strain. Moreover, when the mutant was cultured under the condition of LB + 9.0% NaCl, it could quickly accumulate k+ in the cell and EPS outside the cell."

Morphological changes of Enterobacter cloacae before and after plasma mutagenesis

(Note: A is wild strain; B is mutant strain LB + 9.0% NaCl)

Case 2： High Throughput Screening of Mutant Strains of Oil Producing Yeast by ARTP Combined with 96 Well Plate

ARTP mutation breeding method can quickly and effectively mutate the oil producing yeast rhodosporidium toruloides with good effect. Through the screening of 44 mutagenesis strains, the mutation rate was 47.2%, and the positive mutation rate was 27.2%. A high-throughput screening method based on Nile red staining was established to screen a mutant strain(C4) of high oil and fat, which was cultured in YEPD culture medium for 78h, and its oil production was 2.2 times that of the original strain, and its biomass was increased to 1.5 times that of the original strain.

The relative proliferation rate and relative oil content of primitive bacteria and 44 mutant strains (cultured for 48h)

(The dotted line shows the relative proliferation rate and oil content of the original strain, and the solid line shows the position where the proliferation rate and oil content reach 1.2 times of the control strain)

Case 1：Screening of High Salt Tolerant Mutant Strains by ARTP

"After plasma treatment, when Enterobacter cloacae was cultured in the medium containing 7.5% NaCl, a salt tolerant mutant appeared, and the degradation percentage of TPH was 2.5 times higher than that of the original strain. Moreover, when the mutant was cultured under the condition of LB + 9.0% NaCl, it could quickly accumulate k+ in the cell and EPS outside the cell."

Morphological changes of Enterobacter cloacae before and after plasma mutagenesis

(Note: A is wild strain; B is mutant strain LB + 9.0% NaCl)

Case 2： High Throughput Screening of Mutant Strains of Oil Producing Yeast by ARTP Combined with 96 Well Plate

ARTP mutation breeding method can quickly and effectively mutate the oil producing yeast rhodosporidium toruloides with good effect. Through the screening of 44 mutagenesis strains, the mutation rate was 47.2%, and the positive mutation rate was 27.2%. A high-throughput screening method based on Nile red staining was established to screen a mutant strain(C4) of high oil and fat, which was cultured in YEPD culture medium for 78h, and its oil production was 2.2 times that of the original strain, and its biomass was increased to 1.5 times that of the original strain.

The relative proliferation rate and relative oil content of primitive bacteria and 44 mutant strains (cultured for 48h)

(The dotted line shows the relative proliferation rate and oil content of the original strain, and the solid line shows the position where the proliferation rate and oil content reach 1.2 times of the control strain)